 This study used RNA sequencing and immunohistochemistry staining to identify and invalidate genes associated with gastric cancer, GC, revealing that KLK7 and KLK10 are correlated with differentiation, depth of invasion, and overall survival in GC. Additionally, 10 HUB genes from DGs in GCNAG and 5 HUB genes in IMNAG were demonstrated to have a significant correlation with the prognosis of GC. This article was authored by AI Li, Yen Li, Ya Ya Li, and others.