 In macroatophagy, disc-shaped double membrane structures called phagophores elongate to form cup-shaped structures, which then fuse with lysosomes to create autolisosomes. During this process, the phagophores interact with the endoplasmic reticulum, ER, mitochondria, and other organelles, which suggests that these organelles may be involved in the formation of autophagosomes. Furthermore, the ER remains associated with most autophagosomes and autolisosomes, indicating that the ER may play an important role in autophagy. Additionally, our study shows that phagophores often form near other autophagic structures, suggesting that there may be hotspots for autophagosome formation. Finally, our study reveals that the ER is the most frequently engulfed organelle by both phagophores and autophagosomes, providing further evidence of its involvement in autophagy. This article was authored by Satoru Takahashi, Keiko Saito, Ikuko Koyamahanda, and others.