 Ancient DNA imputation is a reliable method that can improve ancient DNA studies by providing accurate genotypes for low coverage genomes. This study found that when downsampled at 1x, 36 out of 42 genomes were imputed with low error rates, less than 5%, with African genomes having higher error rates. The downstream analysis results between imputed and high coverage genomes were similar, except for the African genomes. This article was authored by Barbara Sousa Diemota, Simone Rubinacci, Diana Ivette Cruz Duvallos, and others.