 Introduction computational analysis of genome or exome sequences may improve inherited disease diagnosis, but is costly and time-consuming.Methods we describe the use of iobio, a web-based tool suite for intuitive, real-time genome diagnostic analyses.Results we used iobio to identify the disease-causing variant in a patient with early infantile epileptic encephalopathy with prior non-diagnostic genetic testing.Conclusions iobio tools can be used by clinicians to rapidly identify disease-causing variants from genomic patient sequencing data.This article was authored by Alistair Ward, Maria Karen, Tonya D. Isera, and others.