 We are pleased to present you a video abstract of a recent paper Integrated Process Design for Bio-Catalytic Synthesis by Eléloir Klaikosl-Trenzphrase, UDP Glucose Production with Sucrose Synthase, to be published in biotechnology and bioengineering. The authors of this work are Dr. Katerina Schmölzer and Martin Lämmerer from the Austrian Center of Industrial Biotechnology Graz Austria, Dr. Alexander Gutmann and Professor Dr. Bernd Niedetzky from the Institute of Biotechnology and Bio-Chemical Engineering at Graz University of Technology Austria. Nucleotide sugar-dependent glycosyl transferases, so-called Léloir glycosyl transferases, represent a new paradigm for the biocatalytic production of glycosides as fine chemicals. However, the process technology for their effective use isn't well established. And we demonstrate in our study of UDP Glucose Production by Sucrose Synthase from Acidityopacillus-Caldus that integrated process development is key to the success of a glycosyl transferase process. We considered the interrelation of all tasks, including the biocatalytic production, the biocatalytic process and the downstream processing right from the beginning. And this was essential to realize UDP Glucose Production at about 100 grams scale. About 60% of the industrial biocatalytic reactions use whole cells for reasons of time and cost. Therefore, the production of recombinant biomass suitable for whole cell catalysis was a clear requirement. And based on the necessary performance metrics of the biotransformation, a specific whole cell activity of 450 units per gram cell drive-it was set as a target value. To achieve this, we replaced E. coli-shakeflask cultivation previously used for production of Suzy AC by a batch fermentation process using LB medium supplemented with glucose, trace elements and additional salts. The time curses of glucose utilization, biomass growths and Suzy AC activity are shown here. The constitutive expression of Suzy AC in E. coli shifted the recombinant protein production mainly to the stationary phase and announced a specific enzyme activity to 480 units per gram cell drive-it. Thus, the target expression level of Suzy AC was clearly reached. In the next step, we applied the whole cell Suzy AC to the synthesis of UDP glucose from sucrose and UDP. A 7-fold molar excess of sucrose was used to maximize the utilization of UDP in the equilibrium controlled reaction of the Suzy. And for high conversion, pH control at 5 was crucial. The UDP glucose production had excellent performance metrics of 100 grams of product per liter, 86% yield based on UDP and a total turnover number of 103 grams of UDP glucose per gram cell drive-it at a space-time yield of 10 grams per liter and hour. We have recently developed a convenient and efficient chromatography-free downstream processing of Indibi sugars from Suzy reaction mixtures. The downstream processing included alkaline phosphatase treatment for selective hydrolysis of the phosphomonoesters UMP and UDP into urodine and inorganic phosphate. Product separation from the remaining sugars, sucrose and fructose and the nucleoside urodine was accomplished by repeated UDP glucose precipitation with ethanol. 30 grams of UDP glucose were isolated in a single batch with 90% purity and in 73% isolated yield. The overall yield based on UDP was 63%. The remaining impurities in the final product were 0.6% of UMP, 6% of inorganic phosphate, 0.7% of sucrose and 1% of ethanol. Overall, the established process would allow the production of 0.7 kilograms of UDP glucose from 1 liter of E. coli bioreactor culture. The success of this holistic approach might support the development of adryglicosyl transferases into industrial bio-catalysts. We want to thank Professor Hans-Jag Weber for NMR measurements and Professor Tom Desmet for the plasmid expression vector with the Suzy AC gene. And thank you for listening to our video abstract on our recent paper.