 Bacillus subtilis strain 168 has undergone significant updates to its functional annotation since its genome sequence was first published 25 years ago. These updates include 1168 genetic functions which were used to construct a new metabolic model of the organism. This model provides insight into the roles of metals in metabolism and macromolecule biosynthesis, as well as the regulation of cell growth and division. Additionally, the model includes proteins that enable accurate classification of cells, allowing for more efficient maintenance and management of the organism. Finally, a comprehensive literature review was conducted to provide further contextual information about the genes and their associated functions. This article was authored by Ahad Braema, Alexandra Kelto, Antoine Danchin, and others.