 This investigation characterizes the fibers extracted from Vachala Mylottica SSP indica tree bark through physical, chemical, thermal, and surface studies. The results show that the fibers have a density of 1.375 gmCm3, with cellulose making up 60.3% of their chemical composition. Wax, moisture, and ash content are present at 0.63%, 6.95%, and 1.12% respectively. FDR spectrum confirms the presence of biopolymers, while X-ray diffraction studies reveal a crystalline index of 57.66%, degree of crystallinity of 70.26%, and crystallite size of 2.27 nanometers. The apparent activation energy of the fibers is 59.17 kW per mole, and they remain stable up to 225 degrees Celsius without degradation. Tensile strength, percent elongation, and Young's modulus are found to be 242 plus or minus 19 mPa, 2.06 plus or minus 0.07%, and 11.76 plus or minus 0.58 gpa respectively. SEM images show a rough fiber surface with average roughness of 56.9668 nanometers, root mean square roughness of 78.5079 nanometers, skewness of 0.45906, and kurtosis of 3.57799 from atomic force microscope images. The study concludes that the physical, chemical, thermal, and surface characteristics of these fibers are comparable to other cellulose fibers. This article was authored by N. Saravanan, P. Genshin, P. Prabha, and others. We are article.tv, links in the description below.