 The prevalence of knockdown resistance, KDR, mutations in the voltage-gated sodium channel, VGSC, gene of 80s albapictus mosquitoes in Shanghai, China has been increasing rapidly over the past few years. This is due to the evolution of mutations in the VGSC gene, which causes the insecticides to become less effective against the mosquitoes. Furthermore, the mutation type F1534i slash ATC, which was previously not observed in Shanghai, was also discovered in the current study. Additionally, the mutation frequency at Kodon 1534 was significantly higher in individuals with intron B compared to those with intron A. Therefore, it is important to monitor the evolution of these mutations and strictly regulate the use of insecticides to ensure the effectiveness of dengue control efforts. This article was authored by Hao Yuan, Wenqi Shan, Yuhang Zheng, and others.