 This new technique, called fast cloning, is a fast, easy, and cost-effective way to clone DNA fragments into vectors or cDNAs. It requires fewer PCR cycles than traditional methods, eliminates the need for gel purification, and reduces the risk of random mutation. Additionally, it can be used for chimera construction, insertion, and multiple mutations in a short stretch of a cDNA. This article was authored by Lugia, Shunbenchang, Wenayun, and others.