Droplet Digital™ PCR Using Bio-Rad's QX100™ ddPCR™ System





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Uploaded on Jun 26, 2012

For more info, visit http://www.bio-rad.com/yt/2/QX100-Dro....
Bio-Rad's state-of the-art Droplet Digital PCR system allows you to quantify target DNA with unrivaled precision by partitioning samples into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are streamed in single file in the QX100 droplet reader. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form.

Conventional real-time PCR can be used to measure only relative amounts of target DNA, whereas the QX100 ddPCR system allows for absolute quantitation of:

• Gene expression
• Copy number variation
• The detection of rare sequences or mutations
• miRNA analysis
• Next-generation sequencing sample quantification


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