 a pure culture study of pickles and acetic acid. Acetic acid bacteria belongs to the family acetylbacteria, which includes several species and genera. The main species that are involved for vinegar production include acetylbacter, glucobacter and gluconoacetobacter. These species have ability to oxidize ethanol to acetic acid and also have ability to the high resistance to acetic acid which is released into the fermentive media. For production of pure culture study of acetic acid, falling procedure is used. We have left grape samples and left the samples for over ripening. After over ripening, 5 cereal dilutions were made by using the similar procedure as described in previous video for cereal dilution preparation. Prepare gyc media for the inoculation of grape samples. This is already prepared gyc media plate for sterilization of media at antibiotic. The solution is prepared by measuring according to 10 mg per ml solution. For addition of antibiotic, we use syringe filter, place the syringe filter and adjust on syringe. Now take antibiotic, mix it properly, inoculate the plate with grape sample from 10 to 5 dilutions. For spreading, place the plate near the flame inoculum evenly throughout the surface of agar. When we distribute the sample on agar surface, inoculum is fully absorbed in the plate at incubator at 30 degree centigrade for 7 days. This is the aceto-vector growth on gyc media. For differentiation of aceto-vector and gluco-vector, we prepared another media known as car media. This is already prepared agar plate with inoculating loop to red hot to cool for 1 to 2 minutes. Now, colunis showing colosone, take those colunis and inoculate on car media by using zigzag manner. For incubation, place the plates at 30 degree centigrade for 4 days. This is the growth of aceto-vector and gluco-vector on car media. The basic differentiation is aceto-vector turns media into green and then into the yellow, whereas gluco-vector remains the media color green. Aceto-vector morphologically characterized and examined according to their acetic acid production with the help of bromocrysol green dye. There are differentiated on the presence of acetic acid production which turns the media color into yellow or green due to the presence of bromocrysol green dye. Aceto-vector turns the media to yellow color whereas gluco-vector turns the media into green.