 Great. Is it okay now? Absolutely. Absolutely. We have, yeah, we have 50 minutes basically subtracting three minutes for the time delay. Go on, please. Thank you. In the name of God, I just, today I'll just speak with you about the effect of the cell-like topography on drug suspectibility of the breast cancer cells to dark stormy. I'm sorry for the interruption because I think you're in the presenter mode. That was great. That was great. Go on. Thank you. Let me introduce myself very short and briefly. I'm a PhD of Bacterial Science and I have worked on surface engineering and modification of biomaterials. Okay. Good. As you know, when the biomaterials implanted the body, you know, it is near the cells. So the surface properties of the biomaterials can affect the cell response. And what I mean is that the response of the host organism in microscopic cellar and protein level to biomaterials is closely associated with the surface properties of the materials. Okay. Effective And in this slide, you can see the effective surface parameters and the biological response of biomaterials. You know that these parameters are wettability, surface charging, and topography, and surface chemistry. But among these, topography has been reported to play an important role in biological response. And you know, until now, the effect of the surface topography on cellular response has been investigated for so many times and for lots of years. And currently, the influence of the micro-nano topography features on the cells has attracted more and more attention. You know, we can use the micro micro-patterns or nano-patterns, but the best one is micro-nano topographical features. And actually, this is because these kinds of the patterns can completely resemble and mimic the nature extracellular matrix and actually ECM, which is around the cells, and cells can interact and reside with them. These platforms have shown great promise on creating a reliable micro environment. And you can see in this slide that there are so many patterns until now can be checked on the cells' behaviors. But to tell the truth, hierarchical micro-nano patterns have great influence and actually they can have an impact on self-functions and even gene expression or cell differentiations or even the inhalation of the tumor cells into the surrounding tissues. Okay, basically, a quantity of platforms have been introduced until now and check the interaction. And the interaction of these patterns with the cells are completely checked. But recently, but the problem is that most of them are not realistic and they are unnatural. But recently, unfortunately recently, selling printing techniques has been proposed as a straightforward and easy and even cost-effective method for fabrication of these kinds of micro-nano patterns, which is called soda-3D patterns or substrate or even some selling printed patterns. In this method, as you can see in the left top part of the slide, in these patterns, in this method, fixed cells are used as templates and to mimic and recapitulate the cells membrane. And actually, these specific biogeomersical features are implored to changing some kinds of the behavior of the cells. You know, you can see that previously it was demonstrated that culturing of cells on the selling printed patterns can change the morphology, adhesion, and even cell differentiation of cells. And in another study, it was also shown that the some cells can even could be re-differentiated or trans-differentiated when culturing on the cell and selling printing substrates. In addition to the changing of some kind of the cells behavior, just like the adhesion and morphology and gene expression, as a proof of concept, also, there is another study which just showed that the morphology and the morphology of the cells and the physical architectures of the cancer cells, actually, could also enhance the clinical activity of some kinds of drugs. And we just use this idea and try to expand this idea to check the effect of the morphography on the viability and even drug susceptibility of the breast cancer cells. You know that the breast cancer is one of the leading and dominant cause of the death in all of the world, not in Latin Iran. And you know, some kinds of the chemotropic drugs, just like doc-sorbasing, have been extensively used. But the problem is that the doc-sorbasing has some kinds of effects. And we just try to optimal the dosage to decrease these kinds of side, in order to decrease these kinds of side effects while maintaining the efficacy. And we're just thinking to use the Gelma Hydrochial as a promising matrix for in vitro-making breast cancer environment. And also PDMS, which is a well-developed substrate and currently used as a cellular studies. Okay. At first step, we just try, we use the cell imprinting to fabricate some soldo-3D substrates. You know, the platform was manufactured by the cell imprinting methods. And I mean that the cell imprinting surface is fabricated on the PDMS and Gelma. And you know, this was the first time that the Gelma was actually the Hydrochial can cell imprinted for the first time. And you know, we use the Gelma, it is worth mentioning to say that we use the Gelma because we can change the stiffness of the Gelma to adjust with the in-wheel tumor, breast tumor. And then we just characterize both of these substrates with the SDM and also AFM to make sure that we can see the patterns of the surface. Okay. At next step, we check the viability and some cellular behavior of the two kinds of breast cancer cells on this cell imprinted substrate. And the results was okay, because the results showed that the breast cancer cells exhibited higher viability and higher attachment and even better growth on the both PDMS and the actually Gelma cell imprinted platform compared with the plain surfaces. You can see the PDMS data here and here are the results of the Gelma substrates, with and without MCF7 patterns and hence also the viability, which is in the right top of the slide. After the proof of concept to elaborate the phenomena, we just use another cancer cell, something like KBR3, and check if when we change the imprinting of the substrates, if the cell's behavior will change or not. And we found that the MCF7 cells had these similar growth properties on MCF7 cell imprinted surface compared to SCA or B3 cell imprinted surfaces. And this was just because of the higher roughness and also larger surface area, which were provided by the MCF7 cell imprinted substrates. And then after assessing the viability, and we just checked if the cells can immigrate and actually located in the pattern or not. And the results showed that for longer time of culturing, for example, five days after culturing, some cells professionally added and spread on the embedded cell-like patterns. So we ensure that the cells are completely located and migrate to imprinted features. So Fatima, we are at the 10-minute mark, so maybe we have two minutes. Okay. And then at last, we just checked the effect of the drug for the different cell-imprinted substrate. And the results showed that the imprinted cell-like topography altered the toxicity effect of the drug. And interestingly, the gelma hydrogel that we used, that we were used, presented better and higher toxicity. And that was great because most of the cells were completely dying. And we just checked for another and get the good results. And now we are going to do another project. We're just thinking to combine the cell-imprinting methods with another methods, which is currently used, which is microfluidic. And then we try to determine the drug efficacy in more and more reliable environment. And in order to target our dreams, we just thinking to follow some steps. At first step, we should try to develop the engineering cell-imprinted microchannels. And then we design and fabricate the microchip with the hypoxic condition. And also we want to define electrode for cell monitoring. And then we check the IC50 in more reliable environment. And at last, we try to culture cells on the microchip and try to check the drug efficacy in more and more reliable environment. Thank you so much. It's gone. Well, thank you very much for the interesting talk. Again, it's great we have such a diverse range of talks today. So we have Yasser waiting for us and another, and we have one question from Mariam, but we have to wait for the break. So let's leave the question for the very, very squeezing speakers for the second round. I'm sorry about that, but let's see how we proceed to the whole session. Yasser, I hear you are online. Can you present? Share your screen.