 Cindi is a program that lets you analyze immunofluorescent images so let's load one of these images. So after you loaded the image here you can specify which channel has the morphology information which channel has the synapse information and which channel has the sort of protein of interest information and if you use LSM files it automatically gets the correct resolution. It will then detect the SOMA of the neuron and then you just press the next button and it detects the new rights in the image. And then when you click next again so it goes step-by-step through these things it detects the synapses and here you can see sort of the synapse profiles. So it detects the synapses and the centers of the synapses and here it shows how does these different channels the red green and blue vary as a function of distance from the synapse center. So once you're done with this you can export the data and you get the information written to file and that's about it. So another little thing I just want to show is that let's say you have a student that's been slaving away for about a week and it's Friday and it's time for them to have their weekly meeting and they recorded many neurons and now they want to analyze it. So what you could do then is let's see where do we have it so let's say they have analyzed many cells and now they quickly want to generate some figures. Possibly that one. So now I loaded four cells and then we can specify these cells let's say are wild type and these cells are some knockout and then directly they can create different graphs and export them to EPS files. So for instance they can get synapse profiles, shot analysis and many other figures just instantly available. And that's that for this minute. Thank you very much.