 We have developed a novel next-generation sequencing, NGS, library preparation method that is suitable for extremely AT-rich genomes. Our method involves using a PCR additive, TMAC, to improve the amplification of highly AT-rich regions and reduce bias towards GC-neutral templates. This development will greatly benefit sequencing clinical samples that often require amplification due to low mass of DNA starting material. This article was authored by Loyola Samuel O, Otto Thomas D, G.U. Young, and others.