 This study aimed to estimate freezing injury using a vegetative index developed from hyperspectral imagery on 6-6 leaf stage canola plants subjected to minus 10 degrees Celsius for six hours and imaged at different thawing times. NDVI and TVI were calculated, and while NDVI increased initially and then decreased at eight hours, TVI gradually decreased with increased duration of thawing. The indices differentiated frozen from control plants and within the freezing treatments, suggesting that recent advancements in low-altitude remote sensing can enable early estimation of frost damage. This article was authored by HEMA-SNJUJU, Vladimir Pajik, Scott D. Nable and others.