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Published on Sep 6, 2011
This series of short chalk-talk videos, provided by Matthias Dobbelstein (Molecular Oncology, GZMB), will cover the principles and calculations associated with quantitative PCR. The second portion of the introductory video addresses the following questions. Why is PCR suitable to determine the quantitative differences of a given template in different samples? What are the limitations set by the saturation phenomenon? And how can even conventional PCR provide some quantitative information?