 This study found that Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Klebsiella pneumoniae were the most commonly isolated pathogens from clinical samples in Punjab, Pakistan. These pathogens were mostly isolated from pus, urine, blood, and sputum. The prevalence of multi-drug resistant strains of these pathogens suggests that current antimicrobial options are severely limited in Pakistan, which poses a serious threat to public health. To address this issue, it is important to implement antimicrobial stewardship programs to ensure prudent prescribing of antibiotics and to reduce inappropriate antimicrobial use. Additionally, it is essential to develop and implement strategies to improve access to quality diagnostics and treatment services, as well as to strengthen surveillance systems to monitor the spread of resistant organisms. This article was authored by Zikria Salim, Abdul Haseeb, Safa S. Al-Marzaki Abhusein, and others.