 The study aimed to investigate the existence of a neuronal pathway from hindbrain appendymocytes to cuspeptin neurons in the arcuate nucleus, arc, and antiroventral periventricular nucleus, AVPV. Wheat germaglutinin, WGA, a transynaptic tracer, was injected into heterozygous kiss1, tandem dimer tomato, TD tomato, rats to visualize cuspeptin neurons. WGA immunoreactivities were found in bimentin immunopositive appendymocytes of the fourth ventricle, IVV, and central canal, CC, as well as in some TD tomato-expressing cells in the arc and AVPV, DBH immunopositive cells in the A1, a 7-neurodenergic nuclei, and CRH immunopositive cells in the PVN. These results suggest that hindbrain appendymocytes have neuronal connections with cuspeptin neurons via hindbrain neurodenergic and CRH neurons to relay low energetic signals for regulation of reproduction. This article was offered by Chakayadura, Shorimeneb, Kana Akigami, and others. We are article.tv, links in the description below.