 The paper discusses the limitations of human mesenchymal stem cells, HMSCs, found in current sources and the need for their in vitro culture expansion. However, the use of supplements containing xenogenic components in the expansion media poses a risk to post-transplantation safety. The paper suggests the necessity to identify and develop chemically defined or humanized supplements to make in vitro-cultured cells safer for transplantation in regenerative medicine. The paper outlines the caveats associated with conventionally used supplements of xenogenic origin and presents possible alternatives, additives that could pave the way for a new era in translating in vitro-cultured cells to therapeutic interventions. This article was authored by Chondinata Cart, Genki Ponro's Gunaseng, K. M. Cherian and others.