 This paper introduces a novel method called positive dialectrophoresis-induced deterministic lateral displacement, PDE-PDLD, flow cytometry. This system uses a combination of positive dialectrophoresis and deterministic lateral displacement to focus and trap single cells in a wide channel, allowing for the collection of Raman spectra from individual cells. This system has been used to analyze the metabolism of various types of cells including yeasts, algae, bacteria, and cancer cells. The system has shown to be able to collect data at rates up to 2,700 events per minute, making it one of the most efficient methods for analyzing metabolites in single cells. Additionally, this system has been shown to be capable of detecting metabolic differences between different cell types, as well as detecting changes in metabolism over time. This article was authored by Xixian Wang, Li Huiren, Zhijian Diao, and others.