 Kytosan is a biocompatible and biodegradable polysaccharide derived from Kytan, which has emerged as a material of promise for drug delivery and biomedical applications. Various extraction methods can produce materials with unique properties, which can be further modified to enhance their bio-activities. Kytosan-based drug delivery systems have been developed for different routes of administration, including oral, ophthalmic, transdermal, nasal, and vaginal, allowing for targeted and sustained release of drugs. Additionally, Kytosan has been used in numerous biomedical applications, such as bone regeneration, cartilage tissue regeneration, cardiac tissue regeneration, corneal regeneration, periodontal tissue regeneration, and wound healing. Furthermore, Kytosan has been utilized in gene delivery, bio-imaging, vaccination, and cosmaceutical applications. Modified Kytosan derivatives have been developed to improve their biocompatibility and enhance their properties, resulting in innovative materials with promising potentials in various biomedical applications. This article was authored by Nimit Desai, Vani Rana, Sagar Salave, and others. We are article.tv, links in the description below.