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Published on Sep 2, 2015
Paola Leonor Barba Burgos - "Genetic dissection of disease resistance and pest related traits in hybrid grapevine families"
Many grapevine breeders seek to combine the fruit quality of Vitis vinifera cultivars with a wide range of adaptive traits from their wild relatives. Among these, resistance to disease is the most representative example. We studied the genetics and genomics of powdery mildew (Erysiphe necator) and phomopsis (Diaporthe ampelina) disease resistance; predatory mite (Phytoseiid) abundance; and trichome trait densities, such as domatia, leaf hairs and bristles; in bi-parental F1 families descending from V. rupestris B38, V. cinerea B9, V. vinifera ‘Chardonnay’, Illinois 547-1 and the complex hybrid ‘Horizon’. We used genotyping-by-sequencing (GBS) to rapidly identify single nucleotide polymorphisms across the whole genome and developed novel methods to generate dense genetic maps. While powdery mildew resistance, predatory mite abundance and trichome traits were found to be controlled by quantitative trait loci, resistance to phomopsis is caused by two single loci with major effects, which are associated with differential expression of NBS-LRR loci among the progeny. Our work provides knowledge of the mechanisms of powdery mildew resistance; phomopsis cane resistance; and plant effects upon the abundance of predatory mites, and delivers tools for the creation of dense genetic maps in heterozygous crosses and for marker assisted selection in grapevine breeding.