 Hello, my name is Eunali in College of Life Science, Kyung University. In case of catalysis injury, self-regeneration is quite limited because of lack of blood vessel and low cell density. Therefore, regeneration of catalysis can get tremendous benefit from tissue engineering abroad. Actually, limited type of tissue can be engineered in vitro, and catalysis is one of them. To construct functional catalysis tissue for transplantation, monitoring of tissue formation is essentially required. However, current monitoring methodologies involve destructive methods such as biochemical osse and histological evaluation. In order to monitor the tissue reconstruction non-destructively, the electric tissue property is a good index to describe the status of tissue formation. And we found that different compositions of catalysis tissue can lead to different electrical tissue. Hello, my name is Dong Hyun-woo. I'm working in Biomedical Engineering Department at Kyung-hee University. The bio-impedance spectrum of tissue can provide information to estimate physiological and pathological conditions of tissue in vivo and in vivo, providing diagnostic medical information as well as expanding our understanding of bioelectromagnetic phenomena. To estimate the electrical tissue property during in vitro control genesis, we developed the new design of bioimpedance spectroscopy system, which used the multiple electrode and localized electrical energy concentration method. We achieved the more sensitive to the typical reasonable interest under the BIS proof, and it could effectively eliminate the geometrical effect and greatly measure the tissue conductivity and its degree of anisotropy. In this paper, we report for measured value at 1 kHz. We are going to research more the spectrum of tissue in vitro control genesis to show frequency dependent characteristic of catalysis tissues. Disc type ionic catalysis were grown in vitro, and measurement was made just by touching the surface of tissue without degenerating effect. Natural articular catalysis shows structural difference according to the depth from the surface. In vitro grown disc type catalysis also somehow get matured and mimic those depth dependent structures to some degree. Many reports including ours show that in vitro control genesis follows some common path in terms of tissue complexity. In our study, the values measured from the top and bottom surface were consistently different during in vitro control genesis, and we propose that this reflects the tissue complexity. The speed of in vitro control genesis may depend on the culture condition, concentration of growth vectors, and the characteristics of cells used. This BIS based monitoring can help to identify the status of tissue formation just by touching the surface of in vitro grown tissue with orade electrodes. Oplandos non-destructive monitoring method to close loop with back control might enable us to produce in vitro reconstructed tissue in consistent quality. Thank you for your attention and please refer to our research article for more detail.