 The study employed mild microwave irradiation with alkaline assistance to pretreat banana peduncle for potential use in various industries. Compositional analysis was conducted at different NaOH concentrations and temperatures, revealing that microwave-assisted alkaline pretreated samples had higher delignification rates, 60.86% at 6.25 N NaOH, compared to alkaline pretreated samples, 48.63% at 7.5 N NaOH. The study also found that microwave-assisted alkaline treatment increased surface exposure, poor volume, and poor diameter in the banana peduncle.