 Severe stress exposure increases the risk of developing major depressive disorder. This is characterized by impaired social functioning and lack of motivation. A chronic social defeat stress model has been developed to study this condition in animals. This model induces a cascade of physiological and behavioral changes. Currently available marclist pose estimation tools enable more complex and naturalistic behavioral tests. DeboF is an open-source tool that provides supervised and unsupervised pipelines for analyzing the behavioral profiles of mice exposed to chronic social defeat stress. The supervised pipeline identifies a distinct stress-induced social behavioral pattern, which is particularly observed during the first encounter and then gradually decreases over time. Additionally, the unsupervised pipeline provides a more detailed profile of the behavioral changes. Furthermore, DeboF aims to facilitate reproducibility and unification of behavioral classification by providing an open-source tool, which can advance the study of rodent individual and social behavior, thereby enabling biological insights and, for example, subsequent drug development for psychiatric disorders. This article was authored by U.E. Boards, Lucas Miranda, Maya Reinhardt, and others. We are article.tv, links in the description below.