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Published on Aug 24, 2012
For more info, visit http://www.bio-rad.com/yt/1/QX100-Dro.... Researchers at Sangamo BioSciences use Bio-Rad's QX100 Droplet Digital PCR system to quantify the level of HIV reservoir in treatment subjects with their unique zinc-finger-based therapy. At approximately one copy of HIV in 1,000 cells analyzed, this quantification would present a challenge for conventional methods.
The Droplet Digital PCR system allows for the quantification of target DNA with unrivaled precision by partitioning samples into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, PCR-positive and PCR-negative droplets are counted to provide absolute quantification of the target DNA.
Presenter: Dr Gary Lee Senior Scientist, Sangamo BioSciences, Inc. Point Richmond, CA