 This study investigates the use of an alkaline protease produced by Bovarier SP, MTCC5184, to de-gum Chinese bivaltine raw silk fibers, achieving complete de-gumming in 45 minutes with 75 units of enzyme per gram of silk at optimal temperature and pH. The obtained fibers were clean, separated, had a smooth fill with shine as compared to untreated fibers. Additionally, serocin isolated from silk cocoon was hydrolyzed with the same alkaline protease to obtain small molecular weight peptides that can be utilized for various applications in cosmetics, pharmaceuticals, and industries. This article was authored by Snell V. Moore, Saqualia Chavon and Osmita A. Prabyan.