 the article presents a new method for molecular cloning that is simple, efficient, and reliable. It involves PCR amplification of the vector and inserts separately, followed by DPNI digestion to eliminate DNA templates used in PCR reactions. The resulting mixture is then transformed into competent E. coli cells to obtain the desired clones. This method does not require gel purification or specialized enzymes, and has many advantages over other cloning methods, including reduced chance of random mutations and high effectiveness and reproducibility. It can also be used for chimera construction, insertion, and multiple mutations in a short stretch of CDNA. This article was authored by Lu Geo, Shen Ben-Chan, Wen Ayun, and others. We are article.tv, links in the description below.