Loading...

MicroRotofor™ Tutorial — Loading the Sample

749 views

Loading...

Loading...

Transcript

The interactive transcript could not be loaded.

Loading...

Loading...

Rating is available when the video has been rented.
This feature is not available right now. Please try again later.
Published on Jun 26, 2012

For more info, visit http://www.bio-rad.com/yt/4/MicroRotofor.
This video tutorial demonstrates sample loading in the focusing chamber of Bio-Rad's MicroRotofor cell. The narrator also shows how to remove air bubbles from the chamber. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/desti...

Loading...

When autoplay is enabled, a suggested video will automatically play next.

Up next


to add this to Watch Later

Add to

Loading playlists...