VCell v5.1 PIP2 to IP3 Tutorial





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Published on Aug 14, 2013

This tutorial models the behavior of a fluorescent biosensor for IP3 release, as occurs during signaling events. The pleckstrin homology (PH) domain binds primarily to cytosolic IP3, but also binds to PIP2 in the membrane and can be tagged with the fluorescent molecule GFP. As PIP2 in the membrane is converted into diacyl glycerol and IP3, PH-GFP translocates from the membrane to the cytosol. This tutorial highlights the use of functions to create simulation results that can be compared to fluorescence images, in this case by creating a "species" that represents the sum of all GFP tagged species in the the cytosol. In addition, this tutorial shows how to add a time-dependent event to stimulate more production of IP3 after reaching an initial steady state, thus increasing the amount of IP3-PHGFP complex and altering the ratio of membrane vs cytosolic fluorescence. Finally, you will create a spatial geometry using analytic expressions.

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