 Pac-Bio hi-fidelity sequencing, hi-fi, has enabled the detection of de novo mutations, DNMs, small indels, short tandem repeats, SDRs, and structural variations, SVs, at unprecedented depth and accuracy. This study demonstrated that hi-fi LRS can accurately identify DNMs of all types, including substitutions, indels, and SVs, with a sensitivity of up to 92%. Additionally, hi-fi LRS can be used to determine the parent of origin of small DNMs, which is important for understanding the genetic basis of diseases. This article was authored by Erdike Cicke, but PGH van der Sanden, Luka Gorman, and others.