 The zebrafish gene ELF3 is essential for proper development of multiple tissues, including the nervous system, muscles, and skin. It is expressed in these tissues throughout development, and its absence leads to malformations in all three systems. These malformations include shortened and bit larvae, defects in the craniofacial cartilage and fins, and neural defects. Additionally, the extracellular matrix, ECM, and the fin and notochord is disorganized, and optic nerve fasciculation, bundling, and arborization in the optic tectum are affected. Furthermore, dysregulation of genes encoding ECM proteins and MMPs may contribute to the observed defects. Overall, this study suggests that ELF3 is necessary for normal development of multiple tissues in zebrafish.