 The study aimed to establish a criterion for identifying when a lipid bilayer has reached steady state using molecular dynamic simulation technique. Two different lipid bilayer systems were simulated in aqueous solution with CACL dollar as electrolyte, and the temporal evolution of lipids properties such as surface area per lipid, deuterium order parameter, lipid hydration, and lipid calcium coordination was studied. The results showed that the hydration of lipids or mean area per lipid are stable indicators of steady state for lipid membranes. This article was authored by Rodolfo de Parasso and Jose J. Lopez Cascales.