 Metabinomics or metabolomics is a branch of science that uses metabolic profiling to study biological systems and predict behavior. It involves analyzing metabolite profiles in biological fluids by two techniques, nuclear magnetic resonance, NMR, spectroscopy and mass spectrometry, MS. The former is often combined with liquid chromatography, LC, to identify metabolites in samples. Despite its potential, metabolite identification can be challenging due to the complexity of the data produced by these techniques. This article provides guidance on methods for identifying metabolites in biological fluids using NMR spectroscopy and illustrates it with examples from recent studies on mice. This article was authored by Anthony C. Dona, Michael Kiriakides, Flora Scott and others.