 The gingival tissue is the target site for certain topical medications, but its permeability has not been systematically evaluated. Pig tissue is commonly used as a model for in vitro membrane transport studies, but it has not yet been determined whether pig tissue can accurately represent human gingivy. This study aimed to assess the permeability of both fresh and frozen human gingivy, as well as pig gingivy, with two different types of permeant molecules. The results indicated that fresh human gingivy had a higher permeability than fresh pig gingivy, while frozen human gingivy had a lower permeability than either fresh or frozen pig gingivy. Additionally, the permeability of frozen pig gingivy increased significantly when stored, indicating that pig gingivy may not be a suitable substitute for human gingivy. This article was authored by Appippa Wanasathap, Hyogen Alex Choi, Pacharoan Namansaphon, and others. We are article.tv, links in the description below.