 The study aimed to develop an effective method for rooting micro-cuttings of apple-neotetroploids using in vitro techniques, optimizing the rooting process by testing different combinations of oxins and putrescene on MS medium with reduced nitrogen content. The results showed that tetraploid micro-cuttings had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts. This article was authored by Maugrajatapadwazinska and Miroslava Cislenska.