 This systematic review evaluates the potential utility of lab-on-a-chip, LOC, technology for antimicrobial susceptibility testing, AST, of Staphylococcus aureus, highlighting its ability to provide rapid access to minimum inhibitory concentration data and aid in selecting appropriate antibiotics and dosages for treating challenging infections caused by this microorganism. This article was authored by Carlos M. Ardila, Mateo Zuluaga Gomez, and Annie Marcella, V-Bars Biles.