 This study demonstrated that clodon subtypes can be used to reconstitute tight junctions in epithelial cells. Clodon 2-10a-10b and minus 15 were found to form paracellular channels when expressed alone, suggesting that they may play important roles in paracellular transport. Additionally, clodon 1-3-4 and minus 7 were shown to be necessary for the formation of functional tight junctions. This study provides insight into the role of clodon subtypes in tight junction assembly and could help researchers better understand how tight junctions regulate paracellular permeability. This article was authored by Mikio Furus, Daikinakatsu, Wendy Hempstock, and others.