 Lack cases are multi-component enzymes that can catalyze the oxidation of a wide variety of substrates using molecular oxygen as the final electron acceptor. They are eco-friendly, versatile bio-catalysts with applications ranging from food additives and beverages to biomedical diagnoses, furniture construction, and biofuel production. Additionally, lack cases have been explored extensively in nanobiotechnology for the development of implantable biosensors and biofuel cells. Furthermore, their ability to break down complex xenobiotic compounds makes them valuable tools in enzymatic remediation. This article was authored by Diana M. Mate and Miguel Alcalde.