 Hello, my name is Daniel Blaisner, welcome to the video abstract of our article. In our article we describe the effects of carbon, nitrogen and phosphorous limitation on biomass composition of the heterotrophic dinoflagellate crypsicodinium cunea. Biomass of crypsicodinium cunea can contain of up to 50% starch, 20% lipids and 10 to 15% proteins and accumulates among other fatty acids large amounts of docosahexinoic acid, a poorly unsaturated fatty acid with health benefits and used as food supplement in human consumption and crypsicodinium cunea is one of the few species used for industrial production of this high-value fatty acid. Previous studies of manipulation of biomass composition were performed in ill-defined media containing yeast extract and it was assumed that nitrogen limitation results in cells, rich in lipids. To investigate the effect of nutrient limitation in detail, we cultured perpsicodinium cunea in batch and continuous flow cultures using a well-defined medium containing glucose or acidic acid as carbon source, Potamic acid as nitrogen source and potassium phosphate as phosphorous source. To conclude, nutrient limitation in continuous flow cultures can be used to manipulate biomass composition of crypsicodinium cunea. Starch contents did decrease when glucose was limited and protein contents decreased when glutamic acid was limiting nutrient. The lipid content however was unaffected by phosphate, glutamic acid or glucose limitation while highest docosahexinoic acid concentration was found in phosphate limited continuous flow cultures. Continuous flow cultures limited in any of these three substrates can therefore be used for production of crypsicodinium cunea, rich in lipids and docosahexinoic acid. Crypsicodinium cunea has also been used as aquaculture feed and therefore we recommend a cultivation under phosphate limitation where starch, lipids and proteins represent at maximal contents. This article gives further information about elemental biomass composition and specific growth and substrate uptake rates of crypsicodinium cunea. Enjoy reading it. Thanks for watching.