 This study biosynthesized gold nanoparticles, BTONPs, using Bamiria tricuspus extract to investigate their anti-inflammatory and skin-moisturizing properties in keratinocytes. BTONPs had no cytotoxic effect on keratinocytes, alleviated the expression and secretion of inflammatory chemokines slash cytokine, regulated NF-Kappa-B and MAPC signaling pathways, and promoted hyaluronic acid production. These results suggest that BTONPs can be used as a promising therapeutic alternative for treating skin inflammation and promoting skin health. This article was authored by T. Hoomai Tran, Rongbo Wang, Whom Kim, and others.