 The animal-derived xenogenic biomaterials used in different surgeries are promising for various applications in tissue engineering. To attain a bioactive extracellular matrix, ECM, that can be safely transplanted, the material must first undergo tissue desellularization. The main objective of this study is to assess the structural integrity, biocompatibility, and potential use of various acellular biomaterials for tissue engineering applications. The Trypsin, Triton-X, TX, and Sodium-Dodesil-Sulfate, SDS, Trypsin plus TX plus SDS protocol was used to effectively eliminate the cellular antigens from the biomaterials while preserving the ECM integrity. The desellularized pericardial tissue, DPP, was found to have significantly increased elasticity compared to the control group. Additionally, the tensile strength of both DPP and desellularized porcine pericardial tissue, DPP, was not affected by the desellularization process. Furthermore, all desellularized tissues were biocompatible with persistent growth of the adipose stem cells. This article was authored by Hussain M. L. Hussaini, Imani Mady, Masahiro Kaneda, and others. We are article.tv, links in the description below.