 As concern the fermented design modules this module deal will deal with the cleaning sterilization and the sampling facilities. We know that if we have a fermenter that have to be used for number of operations and for a continuous use. So just one batch to another batch we need the cleaning. So the small scale reactors are taken apart and then cleaned before being reassembled and then mostly their cleaning is carried out ex-situ. So we just disassemble the whole fermenter and then we utilize them for autoclave and then in other case when there is a large fermenters then we have to clean them in situ that is known as clean in place CIP. So the CIP involve the complete cleaning of not only the fermenter but also all the line sump and dump line system, all the internal component of the system, acid addition system, alkali delivery system, aeration delivery system. So there is all the system should be properly cleaned. So that is why the cleaning and then after the cleaning and before running the fermentation process the sterilization of all the line is very critical. So that is why that should be the provision of that. So in this slide you can see that there is a different lines mostly as concern the sterilization that is mostly in a large scale fermenters happen through the steam. So all the have the control all that is controlled by the different walls, automatic walls and then the supply from you can see here in this slide that the blue color show the inside of the fermenter. So and these are the red line show the steam that having the different valve C, valve A and valve B in this case and in this case. So if the pump A will on then that steam can pass in through side into the fermenter but that is only leader when we have to clean either by to sterilize the fermenter inside. But sometimes this fermenter insides can only be supplied the jacket not inside the fermenter just to raise the temperature. But in case of another you can see that the sometimes the material another. So this is only happen when we say that in case of the cleaning but sometimes we have to check the quality of the product inside. So the sampling provision should be enough for mentors we have to take some samples. So in some case there are the different walls and from where we can get the samples and at the time of the sampling then there should be a proper sterilization otherwise when at the time of the sample collection then there is a risk of contamination. So that is why we should be very critical about the in case of that. So then we can see that by this whole process we can use the different line system. So in designing the fermenter we should be care about all such kind of the provisions for a fermenter.