 This study isolated exosomes from amniotic fluid, saliva, and urine using differential centrifugation on sucrose gradients and identified marker proteins such as CD24, CD9, Anchin-1, and HSP-70. The study also extracted a cyan-A from exosomes, which was protected from enzymatic degradation and used for typing of the CD-20 for single nucleotide polymorphism, RS52812045, and sex determination of the fetus based on the detection of the male-specific ZFY gene product. The study demonstrates that exosomes from body fluids carry a cyanase, which can be analyzed and offers access to the transcriptome of the host organism, making it a minimally invasive procedure for diagnostics. This article was authored by Jonson Johannes WG, Rob Ann Cathleen, Widinga Johannes, and others.