 This particle is about transformation of bacteria with plasmid DNA. Heat shock method is very famous method and it can be easily performed in a lab. In this method through heat the foreign DNA is allowed to penetrate inside the bacterial cell. For performing this experiment we need competent cells and in the previous practical we have already made chemically competent cells and then a foreign DNA. It can be a plasmid vector or another vector. The plasmid vector is usually containing our gene of interest as well as an antibiotic selection marker. So we start our experiment. We need two things first, number one competent cells which are already prepared and stored on eyes or 4 degrees centigrade and number two our DNA of interest which is plasmid in this case. We will mix one microliter of plasmid DNA with the competent cells aliquot already thawed and put on eyes. Then after mixing this mixture is allowed to incubate in eyes for about 20 minutes. Transformation of bacterial cells through heat shock method is called heat shock because we give the transforming cell a shock of 42 degrees centigrade. For this we need water bath. So after incubation of competent cells with the plasmid DNA we will give a shock of about one minute and we need for this we need to put the bacterial cells from eyes into the water bath. For bacteria to survive the heat shock we have added one animal of albibrot and to grow the bacteria we need to put this append off at 37 degrees centigrade for about two hours so that bacteria will multiply and grow. After two hours of incubation of bacterial cells we will spread 100 microliter of the culture on an albiblate. The albiblate should be containing your selection marker like any antibiotic. After spreading of 100 microliter of culture this need to be incubated at 37 degrees centigrade overnight. And after incubation if your plasmid has been transformed you will see colonies. After overnight incubation of a bacterial culture your colonies will appear like this if it has transformed plasmid.