 The study aimed to develop a thermostable Gardasil 9 vaccine, using spray drying and evaluated its immunogenicity and protective efficacy in mice at different storage temperatures. The results showed that the spray dried vaccine was effective against HPV following storage at up to 40 degrees Celsius and the current Gardasil 9 vaccine could be stored and transported at elevated temperatures for up to three months without losing efficacy. This study suggests that a thermostable vaccine could decrease costs associated with cold chain maintenance and increase access and coverage in remote regions of the world.