 The paper aims to illustrate collaboration in the field of single-cell sequencing methods and explore key topics and future directions by conducting bibliometric analyses using site space and viosphere software on publications prior to November 2019 from the Web of Science Core Collection about single-cell sequencing methods. The results show that there was a noticeable increase in publications in 2014, with the United States and high-income countries in Europe contributing to most of the records included. Harvard University, Stanford University, Karolinska Institutes, Peking University, and the University of Washington were the biggest nodes in every cluster of the collaboration network, and SA Taikman, JC Mariani, Auregev, and FC Tang were the top-producing authors. Keywords co-occurrence analysis suggested applications in immunology as a developing research trend. The conclusion is that the global collaboration network was informed and that high-income countries contributed more to the rapidly growth of publications of single-cell sequencing technology with immunology being the next research hotspot and developmental direction. This article was authored by Quan Wang, Kalu Yong, Zhen Zhang, and others. We are article.tv, links in the description below.