 The tumour microenvironment has been shown to be a key factor in the development and progression of cancer. In particular, the availability of certain nutrients can affect the metabolism of cancer cells, leading to distinct metabolic profiles and behaviours. To better understand the role of nutrient availability in cancer, researchers developed a cell culture system called tumour interstitial fluid medium, TIFM. This system mimics the nutrient environment of tumours, allowing researchers to study the metabolism of cancer cells under more realistic conditions. Using TIFM, researchers discovered that arginine biosynthesis is active in cancer cells, which helps them maintain adequate levels of this amino acid even when the surrounding environment is deficient in arginine. Additionally, they found that myeloid-derived arginase activity is responsible for the low levels of arginine in tumours. These findings suggest that understanding the role of nutrient availability in cancer may lead to new therapeutic strategies to target cancer metabolism. This article was authored by Juan J. Apais Saab, Lindsay N. Jerozinski, Patrick Bijonka, and others. We are article.tv, links in the description below.