 Abstract ironone, ION, a cyclic terpenoid compound, has shown great promise for the prevention and treatment of cancer. However, its poor water solubility and low permeability limit its effectiveness. Liposomes are a promising delivery system for ironone due to their ability to increase both solubility and permeability. This study used one, two, diolioil, three, trimethylammonium propane, DOTAP, to enhance the binding of ironone to cancer cells and improve its uptake into helar cells. The addition of DOTAP resulted in larger liposomes, increased membrane fluidity, and higher levels of cellular uptake. These findings suggest that DOTAP can be used to improve the efficacy of ironone in treating cancer. This article was authored by Andengma Atmoko, Fibharem Osmoro, Endre Aweazari, and others.