 This study compared three different methods to estimate evaporation fluxes during simulated summer conditions in a grass-covered lysimeter in the laboratory. Two of these methods could be used to partition total evaporation into transpiration, soil evaporation and interception. A water balance calculation, whereby rainfall, soil moisture and percolation were measured, was used for comparison as a benchmark. A hydrous, 1D model and isotope measurements were used for the partitioning of total evaporation. The isotope mass balance method partitions total evaporation of 3.4 mm per day, mmd-sup-1-sup, into 0.4 mmd-sup-1-sup for soil evaporation, 0.3 mmd-sup-1-sup for interception and 2.6 mmd-sup-1-sup for transpiration, while the hydrous, 1D partitions total evaporation of 3.7 mmd-sup-1-sup, into 1 mmd-sup-1-sup for soil evaporation, 0. This article was authored by S.J. Sutonto, J. Wenninger, A. M. J. Coenders-Garrettz, and others.